Webwhile maintaining stable viability stain fluorescence. BD Horizon™ Fixable Viability Stain 620 is best excited by the Yellow-Green laser (with an excitation maximum of 523 nm), but is also well-excited by the Blue laser. It has a fluorescence emission maximum of 617 nm. Flow cytometric analysis of human Jurkat cells stained with BD Horizon ... WebCan be used with other fluorophores excited by 488 (e.g., FITC or CoraLite488) due to large Stokes shift. Protocol example: PMID 27371595. 7-AAD (7-amino actinomycin D) ... An alternative to fixable viability dyes is cell health assay kits, which use a combination of a viability dye and a DNA binding dye to determine the proportion of live and ...
Human Monocyte STING Activation Flow Cytometry Panel
WebCan be used with other fluorophores excited by 488 (e.g., FITC or CoraLite488) due to large Stokes shift. Protocol example: PMID 27371595. 7-AAD (7-amino actinomycin D) ... An … WebAdd 2.5 uL*of ViaKrome Fixable Viability Dye. Vortex. Incubate at 18-25 °C protected from light for 20 minutes. Add 3 mL of PBS 1X. Centrifuge 5 minutes at 300 g. Aspirate the supernatant. Add 500 μL of PBS 1X / … hills brothers
Zombie Violet™ Fixable Viability Kit - BioLegend
WebPreparation. Zombie NIR™ Fixable Viability kit is composed of lyophilized Zombie NIR™ dye and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 100 µl of DMSO to one vial of Zombie NIR™ dye until fully dissolved. 100 tests = 1 vial of Zombie NIR™ + DMSO, 500 tests = 5 vials of Zombie NIR™ + DMSO. Storage ... WebFlow Cytometry Triton™ X-100 Permeabilization Protocol for Directly Conjugated Antibodies A. Solutions and Reagents ... Prior to fixation and antibody incubation, we recommend adding a fixable viability dye such as the Ghost Dye Violet 510 Fixable Viability Dye #59863 to enable identification and exclusion of dead cells from analysis. WebProduct Details. Preparation. Zombie Violet™ Fixable Viability Kit is composed of lyophilized Zombie Violet™ dye and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 100 µl of DMSO to one vial of Zombie Violet™ dye until fully dissolved. 100 tests = 1 vial of Zombie Violet™ + DMSO, 500 tests = 5 vials of ... hills brothers cappuccino coupons